pdms mold  (Micropoint Technologies)

Journal: Cell Stem Cell

Article Title: Engineered intestinal crypt geometry uncovers YAP1-dependent fetal-to-adult transition

doi: 10.1016/j.stem.2026.01.006

Figure Lengend Snippet: Immature intestinal epithelial cells form engineered tissues when cultured in microwells with in vivo crypt size (A) Strategy for culturing engineered tissues. W, WNT; E, epidermal growth factor (EGF); N, Noggin; R, R-spondin 1. (B) Polydimethylsiloxane (PDMS) template used for fabricating bioengineered hydrogel substrates with a range of microwell sizes imitating in vivo crypt sizes. (C) Bright-field image of engineered tissues at day 3 cultured on a scaffold containing a range of microwell sizes. Scale bar, 100 μm. (D) Bright-field (left) and mTomato fluorescence (right) experiments depicting the formation of tissues with distinct morphologies. Scale bars, 20 μm (outset) and 5 μm (inset). Results in (C) and (D) are representative of at least three independent experiments. (E and F) Quantification of lumen formation (E) and columnar cell shapes (F) across tissues with a range of microwell sizes. Data presented in (E) and (F) are means ± SD from three independent experiments. (G) Bioengineered scaffold with 50 μm diameter size. Scale bars, 50 μm (outset) and 20 μm (inset). (H) Bright-field image of an engineered tissue at day 3 cultured on a scaffold with a 50 μm diameter size. Scale bars, 50 μm (outset) and 20 μm (inset). See also .

Article Snippet: This wafer was then used for polydimethylsiloxane (PDMS) molding (Sylgard 184, Dow Corning).

Techniques: Cell Culture, In Vivo, Fluorescence